Very Low-Frequency Broadband Electron Paramagnetic Resonance Spectroscopy of Metalloproteins
نویسندگان
چکیده
A previously developed spectrometer for broadband electron paramagnetic resonance (EPR) spectroscopy of dilute randomly oriented systems has been considerably modified to extend the frequency reach down hundred MHz range and boost concentration sensitivity by 1 2 orders magnitude. The instrument is now suitable study biological in particular metalloproteins. As a proof concept, examples from class low-spin ferric hemoproteins are studied terms frequency-dependent changes their EPR spectra. Mono-heme cytochrome c determined g-strain over wide range, whereas combination unresolved ligand hyperfine interaction concentration-dependent intermolecular dipolar becomes dominant at very low frequencies. In four heme containing c3, combines with intramolecular full-studied 0.23–12.0 GHz. It concluded that point-dipole approach inappropriate describe magnetic interactions between body literature on redox multi-heme proteins will be affected this conclusion.
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ژورنال
عنوان ژورنال: Journal of Physical Chemistry A
سال: 2021
ISSN: ['1089-5639', '1520-5215']
DOI: https://doi.org/10.1021/acs.jpca.1c01217